NF-κB promotes the stem-like properties of leukemia cells by activation of LIN28B

Figure 2 Overexpression of nuclear factor kappa B (NF-κB) in HEK293T cells. A: HEK293T cells were co-transfected with 0.5 μg of pGL3.0, 2 μg of pCMV6-XL4 NF-κB1, 2 μg of NF-κB reporter gene and 10 ng of Renilla vector as indicated on the x-axis in 1mL of DMEM growth medium for 24 h prior to lysis for measuring the luminescence. All samples were normalized with renilla luciferase to ensure equal transfection efficiency. Cells transfected with PGL3 vector was used as negative control. The experiments were repeated 6 times (mean ± SD); B: Western blot analysis of LIN28B protein level in HEK293 cells receiving empty vector and NFκB1 vector as indicated. Proteins were extracted after 48 h of transfection. GAPDH was used as loading control.^aP < 0.05.