Functional and molecular mechanism of intracellular pH regulation in human inducible pluripotent stem cells

Figure 4 Functional characterization of the acid loader. A-D: The traces shown in A and C showed typical pH_i recovery slopes after Na-acetate prepulse-induced intracellular alkalization in HEPES and 5% CO₂/HCO₃^--buffered solution as a control. The traces shown in B and D showed the effect of the removal of extracellular Cl^- (Cl^--free) on the pH_i recovery slope in HEPES and 5% CO₂/HCO₃^--buffered solution; E: The curve of the pH_i recovery rates in HEPES and 5% CO₂/HCO₃^--buffered solution were collected from 3-4 similar experiments shown in A and C. Error bars represent the mean ± SE.