Rat model of anal sphincter injury and two approaches for stem cell administration

Figure 3 Basal anal manometry. A and B: Anal pressure data demonstrate spontaneous rhythmic contractions in the form of “plateaus”, with multiple peaks. At least 30 min were registered and a segment of 12 min with the highest stability was selected. For these tracings selections, we calculated the area-under-the-contraction waveforms vs time curve related to the registry baseline. In A, it is the area between the purple and the red lines. To detect minor changes, we selected the six best contractions; for each one of these we determined (B): total duration (D), time to peak (Tmax), pressure range (range, R), and number of peaks. A mean value of these variables was calculated in each registry.