The famous versus the inconvenient - or the dawn and the rise of 3D-culture systems

Figure 2 Primary human hepatocytes and Hep G2 hepatoma cells 5 d and 24 h after cell seeding into r- (A) and cf-KITChips (B) respectively (upper panels: top view, lower panels: cross section). The r-KITChip (20 mm × 20 mm in total) is comprised of up to 625 round microcontainers (diameter up to 300 μm, depth up to 300 μm) or 1156 cubic microcontainers (300 μm × 300 μm × 300 μm in w × l × h) for the cf-KITChip of which 5 × 5 can be seen in 2A and 4 × 4 can be seen in 2B. Live cell staining with Syto 16. Scale bar: 250 μm.