miR-10a-5p敲低通过靶向THBS2抑制胃癌细胞的生长和转移

图5 过表达THBS2逆转miR-10a-5p敲低对MGC-803细胞增殖, 集落形成, 迁移与侵袭的抑制作用. miR-10a-5p inhibitor和pcDNA-THBS2共转染入MGC-803后, A: Western blot法检测细胞中THBS2蛋白表达; B: RT-qPCR法检测细胞中THBS2 mRNA表达, 与NC+pcDNA-Con组相比, ^aP<0.05; 与miR-10a-5p inhibitor+pcDNA-Con组相比, ^cP<0.05, n = 3; C: CCK-8法检测细胞活性, 与NC+pcDNA-Con组相比, ^aP<0.05, ^bP<0.01; 与miR-10a-5p inhibitor+pcDNA-Con组相比, ^cP<0.05, ^dP<0.01, n = 3; D、E: 集落形成实验检测细胞的集落形成, 与NC+pcDNA-Con组相比, ^aP<0.05; 与miR-10a-5p inhibitor+pcDNA-Con组相比, ^cP<0.05, n = 3; F-H: Transwell法检测细胞的迁移与侵袭能力; 与NC+pcDNA-Con组相比, ^aP<0.05; 与miR-10a-5p inhibitor+pcDNA-Con组相比, ^cP<0.05, n = 3. RT-qPCR: 实时荧光定量PCR.