基于RNA-Seq技术的先天性巨结肠转录组学

表2 3对肠组织样本测序数据与人类基因组参考序列比对情况

样品名称	H0129_BB	H0127_BB	H0116_BB	H0129_ZC	H0127_ZC	H0116_ZC
Total reads	26953378	27594122	31466683	31402169	32446763	23766915
Total mapped reads	20869303	22462832	24245772	24920590	23963792	21364363
Uniquely mapped reads	20174846	21835711	23422760	24129028	23065295	20073384
Multiple mapped reads	694457	627121	823012	791562	898497	1290979
Total mapping rate	77.43%	81.40%	77.06%	79.36%	73.86%	89.89%
Uniquely mapping rate	74.85%	79.13%	74.44%	76.84%	71.09%	84.46%
Multiple mapping rate	2.58%	2.27%	2.62%	2.52%	2.77%	5.43%

BB: 病变肠组织样本; ZC: 正常肠组织样本.