CX3CR1对骨髓间充质干细胞修复受损肠上皮细胞的影响

图7 各个实验组中Caco-2细胞的ZO-1、Occludin蛋白和mRNA的表达. A: ZO-1和Occludin的Western blot条带; B: ZO-1和Occludin蛋白的相对表达量; C: ZO-1 PCR扩增曲线; D: ZO-1 PCR溶解曲线; E: Occludin PCR扩增曲线; F: Occludin PCR溶解曲线; G: ZO-1和Occludin mRNA的相对表达量. 1: Caco-2对照组; 2: Caco-2+BM-MSCs共培养组; 3: Caco-2+TNF-α损伤组; 4: Caco-2+TNF-α+BM-MSCs损伤修复组; 5: Caco-2+TNF-α+anti-CX3CR1-BM-MSCs阻断组. ^aP<0.05 vs 损伤组; ^cP<0.05 vs 损伤修复组. TNF-α: 肿瘤坏死因子-α; BM-MSCs: 骨髓间充质干细胞.