Copyright
©The Author(s) 2014.
世界华人消化杂志. 2014-08-18; 22(23): 3396-3402
在线出版 2014-08-18. doi: 10.11569/wcjd.v22.i23.3396
在线出版 2014-08-18. doi: 10.11569/wcjd.v22.i23.3396
表1 IGF-ⅠR shRNA真核表达质粒及阴性对照质粒序列
| 名称 | 正、反义链(5'→3') |
| IGF-ⅠR-shRNA1 | 5'-CACCGCTGATGTGTACGTTCCTGATTTCAAGAGAATCAGGAACGTACACATCAGCTTTTTTG-3' |
| 5'-GATCCAAAAAAGCTGATGTGTACGTTCCTGATTCTCTTGAAATCAGGAACGTACACATCAGC-3' | |
| IGF-ⅠR-shRNA2 | 5'-CACCGCGTGAGAGGATTGAGTTTCTTTCAAGAGAAGAAACTCAATCCTCTCACGCTTTTTTG-3' |
| 5'-GATCCAAAAAAGCGTGAGAGGATTGAGTTTCTTCTCTTGAAAGAAACTCAATCCTCTCACGC-3' | |
| IGF-ⅠR-shRNA3 | 5'-CACCGCAAAGTCTTTGAGAATTTCCTTCAAGAGAGGAAATTCTCAAAGACTTTGCTTTTTTG-3' |
| 5'-GATCCAAAAAAGCAAAGTCTTTGAGAATTTCCTCTCTTGAAGGAAATTCTCAAAGACTTTGC-3' | |
| IGF-ⅠR-shRNA4 | 5'-CACCGGACAGATCCTGTGTTCTTCTTTCAAGAGAAGAAGAACACAGGATCTGTCCTTTTTTG-3' |
| 5'-GATCCAAAAAAGGACAGATCCTGTGTTCTTCTTCTCTTGAAAGAAGAACACAGGATCTGTCC-3' | |
| Negative-shRNA | 5'-CACCGTTCTCCGAACGTGTCACGTTTCAAGAGAACGTGACACGTTCGGAGAATTTTTTG-3' |
| 5'-GATCCAAAAAATTCTCCGAACGTGTCACGTTCTCTTGAAACGTGACACGTTCGGAGAAC-3' |
引文著录: 严晓娣, 时运, 钱琦, 李景源, 陈心, 董志珍, 姚登福. shRNA干预IGF-ⅠR活化对肝癌细胞增殖的抑制作用. 世界华人消化杂志 2014; 22(23): 3396-3402