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©The Author(s) 2011.
世界华人消化杂志. 2011-02-18; 19(5): 457-466
在线出版 2011-02-18. doi: 10.11569/wcjd.v19.i5.457
在线出版 2011-02-18. doi: 10.11569/wcjd.v19.i5.457
表1 实时荧光定量PCR引物列表
| 引物名称 | 序列号 | 引物序列 |
| SREBP-1a | NM_011480 | 5'-ATGGACGAGCTGGCCTTCGGTGAGGCGGC-3' |
| 5'-CAGGAAGGCTTCCAGAGAGGA-3' | ||
| SREBP-1c | NM_011480 | 5'-GCTGTTGGCATCCTGCTATC-3' |
| 5'-TAGCTGGAAGTGACGGTGGT-3' | ||
| SREBP-2 | U12330 | 5'-AGCTGGCAAATCAGAAAAACAAG-3' |
| 5'-GATTAAAGTCTTCAATCTTCAAGTCCAC-3' | ||
| LXRα | AJ132601 | 5'-TCAGCATCTTCTCTGCAGACCGG-3' |
| 5'-TCATTAGCATCCGTGGGAACA-3' | ||
| LXRβ | NM_009473 | 5'-AAGCAGGTGCCAGGGTTCT-3' |
| 5'-TGCATTCTGTCTCGTGGTTGT-3' | ||
| PPARα | NM_011144 | 5'-TGAGGAAGCCGTTCTGTGAC-3' |
| 5'-GGTGTCATCTGGATGGTTGC-3' | ||
| PPARβ/δ | NM_011145 | 5'-GCCTCGGGCTTCCACTAC-3' |
| 5'-AGATCCGATCGCACTTCTCA-3' | ||
| PPARγ | NM_011146 | 5'-CCCCTGCTCCAGGAGATCTAC-3' |
| 5'-GCAATCAATAGAAGGAACACGTTGT-3' | ||
| HMG-CoA reductase | X00494 | 5'-AGATACTGGAGAGTGCCGAGAAA-3' |
| 5'-TTTGTAGGCTGGGATGTGCTT-3' | ||
| HMG-CoA synthase | L00326 | 5'-CCTGGGTCACTTCCTTTGAATG-3' |
| 5'-GATCTCAAGGGCAACGATTCC-3' | ||
| LDLR | NM_010700 | 5'-CCAACCTGAAGAATGTGGTG-3' |
| 5'-CAGGTCCTCACTGATGATGG-3' | ||
| FAS | NM007988 | 5'-CACAGATGATGACAGGAGATGG-3' |
| 5'-TCGGAGTGAGGCTGGGTTGAT-3' | ||
| SCD-1 | NM009127 | 5'-TGGGTTGGCTGCTTGTG-3' |
| 5'-GCGTGGGCAGGATGAAG-3' | ||
| ACC | AF356089 | 5'-ACACTGGCTGGCTGGACAG-3' |
| 5'-CACACAACTCCCAACATGGTG-3' | ||
| PEPCK | NM_011044 | 5'-GAGTTCCTGCCTCTCTCCAC-3' |
| 5'-TTCCACGAACTTCCTCACTG-3' | ||
| G-6-pase | U00445 | 5'-CATCAATCTCCTCTGGGTGGC-3' |
| 5'-TGTTGCTGTAGTAGTCGGTGTCC-3' | ||
| PGC-1α | BC066868 | 5'-TCTGGAACTGCAGGCCTAACTC-3' |
| 5'-GCAAGAGGGCTTCAGCTTTG-3' | ||
| CYP7A1 | NM_007824 | 5'-AGCAACTAAACAACCTGCCAGTACTA-3' |
| 5'-GTCCGGATATTCAAGGATGCA-3' | ||
| Gck | NM_010292 | 5'-AGAGCAGATCCTGGCAGAGT-3' |
| 5'-TGGTTCCTCCCAGGTCTAAG-3' | ||
| Acox | NM_053115 | 5'-CCAGGACAGAGGTTCTTGGT-3' |
| 5'-TCTCAGGAAGGACTTGGCTT-3' | ||
| Cpt1 | NM_013495 | 5'-CTCAGTGGGAGCGACTCTTCA-3' |
| 5'-GGCCTCTGTGGTACACGACAA-3' | ||
| Acadm | NM_007382 | 5'-TGACGGAGCAGCCAATGA-3' |
| 5'-ATGGCCGCCACATCAGA-3' | ||
| LPL | NM_008509 | 5'-GCCCAGCAACATTATCCAGT-3' |
| 5'-GGTCAGACTTCCTGCTACGC-3' | ||
| FAT / CD36 | NM_007643 | 5'-GCCAAGCTATTGCGACATGA-3' |
| 5'-GATAGACCTGCAAATGTCAGAGGAA-3' | ||
| aP2 | NM_024406 | 5'-AACACCGAGATTTCCTT-3' |
| 5'-ACACATTCCACCACCAG-3' | ||
| β-actin | AY618569 | 5'-AGAGGGAAATCGTGCGTGAC-3' |
| 5'-CAATAGTGATGACCTGGCCGT-3' |
引文著录: 李国生, 刘栩晗, 黄澜, 朱华, 刘亚莉, 马春梅. 2型糖尿病中国地鼠脂肪肝相关胰岛素抵抗的形成机制. 世界华人消化杂志 2011; 19(5): 457-466