延边地区朝鲜族乙型肝炎病毒感染者病毒基因型的检测与分析

表1 引物序列

基因型	PCR	正义链	反义链
A-F	1stPCR	P1: 5'-TCACCATATTCTTGGGAACAAGA-3'	S4R:5'-AGAAGATGAGGCATAGCAGC-3'
A	2ndPCR	B2: 5'-GGCTCMAGTTCMGGAACAGT-3'	BA1R:5'-CTCGCGGAGATTGACGAGATGT-3'
B		B2: 5'-GGCTCMAGTTCMGGAACAGT-3'	BB1R:5'-CAGGTTGGTGAGTGACTGGAGA-3'
C		B2: 5'-GGCTCMAGTTCMGGAACAGT-3'	BC1R:5'-GGTCCTAGGAATCCTGATGTGT-3'
D		BD1: 5'-GCCAACAAGGTAGGAGCT-3'	B2R:5'-GGAGGCGGATYTGCTGGCAA-3'
E		BE1: 5'-CACCAGAAATCCAGATTGGGACCA-3'	B2R:5'-GGAGGCGGATYTGCTGGCAA-3'
F		BF1: 5'-GYTACGGTCCAGGGTTCACA-3'	B2R:5'-GGAGGCGGATYTGCTGGCAA-3'

M: A或C; Y: C或T.