幽门螺杆菌与胃上皮细胞不同作用时间点蛋白质组的差异分析

表1 2-DE图谱差异点的质谱鉴定结果

Spot No.	Accession No.	Protein	PI/MW(Da)
0.5 h-143	gi5644643	chaperonin GroEL (H. pylori)	5.55/58 227.5
0.5 h-200	gi114644226	mitochondrial ATP synthase, H+ transporting F1 complex beta subunit (Homo sapiens)	4.95/48 083
0.5 h-290	gi164451511	actin	5.3/41 796.9
0.5 h-284	gi157111829	actin	5.29/41 615.8
0.5 h-193	gi90077396	unnamed protein product	8.46/29 151.2
0.5 h-478	gi15644820	succinate dehydrogenase iron-sulfur subunit (H. pylori 26695)	5.34/27 633.4
0.5 h-513	gi55775699	V protein [Simian parainfluenza virus 5]	7.55/23 920
0.5 h-517	gi114565485	peroxiredoxin 6 isoform 1 (Pantroglodytes)	5.69/23 205.2
0.5 h-547	gi54036561	cytochrome b-c1 complex subunit Rieske	8.78/29 746.5
0.5 h-579	gi11139093	GrpE-like protein cochaperone (Homo sapiens)	8.29/24 132.9
0.5 h-578	gi15646170	alkyl hydroperoxide reductase(H. pylori 26695)	5.88/22 221.4
0.5 h-689	gi15611830	thioredoxin (H. pylori J99)	5.16/11 847.1
4 h-205	gi89574029	mitochondrial ATP synthase, H+ transporting F1 complex beta subunit (Homo sapiens)	5.8/57 919.4
4 h-289	gi1703127	actin, cytoplasmic type 8	5.31/41 820.8
4 h-287	gi1703123	actin, cytoplasmic type 5	5.3/ 41 822.8
4 h-399	gi149029725	nascent-polypeptide-associated complex alpha	4.87/15 006.8
4 h-400	gi149029725	nascent-polypeptide-associated complex alpha	4.87/15 006.8
4 h-515	gi55775699	V protein [Simian parainfluenza virus 5]	7.55/23 920
4 h-466	gi15645574	hypothetical protein HP0958 (H. pylori 26695)	8.73/29 673.6
4 h-724	gi54301401	alkyl hydroperoxide reducatase (H. pylori)	5.88/22 222.4
4 h-600	gi15646170	alkyl hydroperoxide reductase (H. pylori 26695)	5.88/22 221.4
4 h-652	gi18482498	non-heme ferritin protein (H. pylori)	5.51/19 242.6
4 h-711	gi15611830	thioredoxin (H. pylori J99)	5.16/11 847.1
4 h-599	gi15646170	alkyl hydroperoxide reductase (H. pylori 26695)	5.88/22 221.4
4 h-604	gi15646170	alkylhydroperoxide reductase (H. pylori 26695)	5.88/22 221.4
4 h-559	gi54036561	cytochrome b-c1 complex subunit Rieske	8.78/29 746.5
4 h-535	gi15645038	putative neuraminyllactose-binding hemagglutinin homolog (hpaA) (H. pylori 26695)	5.19/53 905.2
4 h-520	gi15644703	bifunctional urease subunit gamma/beta (H. pylori 26695)	8.46/26 522.9
4 h-617	gi54301431	alkyl hydroperoxide reducatase (H. pylori)	6.08/22 221.5
4 h-666	gi13937981	peptidylprolyl isomerase A (cyclophilin A) (Homo sapiens)	7.68/17 999.9
4 h-656	gi15645018	adhesin-thiol peroxidase (H. pylori 26695)	7.68/18 280.8
4 h-634	gi15645732	amma-glutamyltranspeptidase (H. pylori 26695)	9.27/61 112.9
2 h-6041	gi15646170	alkyl hydroperoxide reductase (H. pylori 26695)	5.88/22 221.4
2 h-760	gi15611830	thioredoxin (H. pylori J99)	5.16/11 847.1

0.5 h-143表示共孵育0.5 h的2-DE蛋白点id值143号的蛋白点, 其他用同样的原则命名.