下调XIAP表达增强化疗药物诱导胃癌细胞凋亡的作用

图1 反义表达载体介导胃癌细胞XIAP表达下调. A: XIAP反义表达载体pcDNA3.1-AS-XIAP, 1, 6: λDNA/HindⅢ + EcoRⅠmarker; 2: BamHⅠ+XbaⅠ; 3: XbaⅠ; 4: BamHⅠ; 5: 空白; B: RT-PCR, 1: 100 bp PCR marker; 2: MKN-45; 3: MKN-45/neo; 4: MKN-45/AS-XIAP; C: Western blot, 1: MKN-45; 2: MKN-45/neo; 3: MKN-45/AS-XIAP