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©The Author(s) 2007.
世界华人消化杂志. 2007-06-08; 15(16): 1859-1864
在线出版 2007-06-08. doi: 10.11569/wcjd.v15.i16.1859
在线出版 2007-06-08. doi: 10.11569/wcjd.v15.i16.1859
表1 HBV DNA基因分型巢式PCR引物序列
| 基因型外引物 | |
| Ps1 5'-TCACCATATTCTTGGGAACAAGA-3' | |
| Ps2 5'-CGAACCACTGAACAAATGGC-3' | |
| 基因型内引物A组(扩增HBV基因A、B、C 型) | |
| B2 5'-GGCTCCAGTTCCGGAACAGT-3' | |
| BA1R 5'-CTCGCGGAGATTGACGAGATG-3' | |
| BB1R 5'-CAGGTTGGTGAGTGACTGGAG-3' | |
| BC1R 5'-GGTCCTAGGAATCCTGATGTTG-3' | |
| 基因型内引物B组(扩增HBV基因D、E、F 型) | |
| BD1 5'-GCCAACAAGGTAGGAGCT-3' | |
| BE1 5'-CACCAGAAATCCAGATTGGGACCA-3' | |
| BF1 5'-GCTACGGTCCAGGGTTACCA-3' | |
| B2R 5'-GGAGGCGGATATGCTGGCAA-3' | |
| HBV 基因亚型 | |
| BJ基因亚型 | |
| 1stPCR 5'-ATGCAACTTTTTCACCTCTGACTA-3' | |
| 5'-ATAGGGGCATTTGGTGGTCT-3' | |
| 2stPCR 5'-ATGCAACTTTTTCACCTCTGACTA-3' | |
| 5'-TTCTTTATAMGGGTCAATGTCCATG-3' | |
| Ba基因亚型 | |
| 1stPCR 5'-ATGCAACTTTTTCACCTCTGCTTA-3' | |
| 5'-ATAGGGGCATTTGGTGGTCT-3' | |
| 2stPCR 5'-TAGCAACTTTTTCACCTCTGCTTA-3' | |
| 5'-TTCTTTATAMGGGTCAATGTCCATG-3' | |
| C1基因亚型 | |
| 5'-TGTTTGGCTTTCAGTTATATG-3' | |
| 5'-AAAGGCATTAATGCTGGGT-3' | |
| C2基因亚型 | |
| 5'-TGTTTGGCTTTCAGTTATATG-3' | |
| 5'-CAAGGCAGTTTTCGAAAACAT-3' |
引文著录: 钟崇芳, 郝娃, 李卓, 孟欣, 严艳, 牛京勤, 殷继明. 乙肝病毒基因型与肝脏病理改变的关系. 世界华人消化杂志 2007; 15(16): 1859-1864