Gastric Cancer
Copyright ©2007 Baishideng Publishing Group Co.
World J Gastroenterol. Dec 28, 2007; 13(48): 6506-6511
Published online Dec 28, 2007. doi: 10.3748/wjg.v13.i48.6506
Table 1 Primers and PCR conditions
mRNAPrimer (5’-3’)Primer size (bp)PCR conditions
CyclinD1F: TGGATGCTGGAGGTCTGCGAGGAA57395°C/3 min;
R: GGCTTCGATCTGCTCCTGGCAGGC
GAPDHF: GAAGGTGAAGGTCGGAGT320(94°C/30 s; 55°C/30 s; 72°C/60 s) 35 cycles
R: GA AGATGGTGATGGGATTTA