Effect of intestinal lymphatic circulation blockage in two-hit rats

Table 1 Effect of mesenteric lymph duct ligation on biochemical indexes of two-hit rats (mean ± SD)

Group	n		AST (nkat/L)		ALT (nkat/L)		BUN (mmol/L)		Cr (μmol/L)		LDH-1 (nkat/L)
	Pre-exp	Post-exp	Pre-exp	Post-exp	Pre-exp	Post-exp	Pre-exp	Post-exp	Pre-exp	Post-exp	Pre-exp	Post-exp
Sham	15	15	1767 ± 333	1917 ± 550	533 ± 150	583 ± 283	7.4 ± 1.0	8.5 ± 1.6	47.7 ± 22.1	50.9 ± 31.7	4334 ± 2033	4051 ± 2567
Non-ligation	15	8	1817 ± 633	5801 ± 1900bd	550 ± 133	4117 ± 1450bd	7.5 ± 0.9	21.8 ± 3.5bd	47.5 ± 18.9	213.9 ± 81.2bdf	4017 ± 2017	7985 ± 2267bd
Ligation	15	11	1667 ± 483	5184 ± 2300bd	500 ± 167	2967 ± 1217bdf	7.8 ± 1.1	15.5 ± 5.6bdf	43.3 ± 18.4	142.0 ± 66.1abd	4917 ± 1767	7151 ± 2250bd

^bP < 0.01 vs pre-exp;

^dP < 0.01 vs sham;

^aP < 0.05,

^fP < 0.01 vs non-ligation.

Table 2 Effect of mesenteric lymph duct ligation on NO₂^-/NO₃^-, NOS and iNOS in serum of two-hit rats (mean ± SD)

Group	n		NO2-/NO3- (mmol/L)		NOS (nkat/L)		iNOS (nkat/L)
	Pre-exp	Post-exp	Pre-exp	Post-exp	Pre-exp	Post-exp	Pre-exp	Post-exp
Sham	15	15	84.2 ± 48.4	79.4 ± 33.3	245 382 ± 132 026	219 544 ± 78 349	87 851 ± 43 842	71 681 ± 37 647
Non-ligation	15	8	88.6 ± 38.5	217.1 ± 48.1bd	251 550 ± 69 347	389 411 ± 90 851bd	91 685 ± 22 338	175 035 ± 45 009bd
Ligation	15	11	86.3 ± 41.7	159.9 ± 44.1bdf	265 053 ± 102 520	312 562 ± 129 193a	85 017 ± 42 842	109 355 ± 24 005f

^bP < 0.01 vs pre-exp;

^aP < 0.05,

^dP < 0.01 vs sham;

^fP < 0.01 vs non-ligation.

Table 3 Effect of mesenteric lymph duct ligation on NO₂^-/NO₃^-, NOS and iNOS in homogenate of two-hit rats (mean ± SD)

Group (n)		Intestine	Kidney	Liver	Lung	Heart
NO2-/NO3- (μmol/g)	Sham (15)	3.3 ± 1.0	1.4 ± 0.3	1.6 ± 1.0	1.4 ± 0.5	2.0 ± 0.4
	Non-ligation (8)	9.1 ± 2.9b	5.1 ± 1.4b	4.0 ± 1.2b	3.2 ± 0.9b	3.5 ± 0.9b
	Ligation (11)	4.3 ± 2.2d	2.9 ± 0.7bd	2.2 ± 0.8d	2.7 ± 0.7a	3.1 ± 0.6a
NOS (nkat/g)	Sham (15)	20.7 ± 4.7	11.2 ± 4.5	12.2 ± 4.3	0.62 ± 0.17	24.8 ± 3.7
	Non-ligation (8)	30.2 ± 9.7a	15.5 ± 6.3b	18.8 ± 8.2	0.84 ± 0.32	28.5 ± 5.1
	Ligation (11)	27.0 ± 6.8	13.7 ± 4.5	13.5 ± 3.8	0.73 ± 0.15	26.5 ± 5.1
iNOS (nkat/g)	Sham (15)	9.8 ± 2.2	6.2 ± 2.8	5.8 ± 2.8	4.0 ± 1.0	11.0 ± 4.0
	Non-ligation (8)	15.3 ± 3.8b	7.7 ± 3.3	8.0 ± 5.0	7.0 ± 3.2	15.2 ± 6.0
	Ligation (11)	12.0 ± 2.3	7.0 ± 4.0	7.0 ± 3.5	5.7 ± 1.3	12.0 ± 4.0

^aP < 0.05,

^bP < 0.01 vs sham;

^dP < 0.01 vs non-ligation.

Table 4 Effect of mesenteric lymph duct ligation on SOD and MDA in serum of two-hit rats (mean ± SD)

Group	n		SOD (nkat/L)		MDA (μmol/L)
	Pre-exp	Post-exp	Pre-exp	Post-exp	Pre-exp	Post-exp
Sham	15	15	1 732 013 ± 134 027	1 687 004 ± 100 020	6.01 ± 1.53	6.82 ± 1.17
Non-ligation	15	8	1 711 342 ± 139 195	1 394 446 ± 210 542bd	5.55 ± 1.18	10.06 ± 1.28bd
Ligation	15	11	1 664 666 ± 116 690	1 610 489 ± 149 197f	6.46 ± 1.48	8.52 ± 1.16bdf

^bP < 0.01 vs Pre-exp;

^dP < 0.01 vs sham;

^fP < 0.01 vs non-ligation.

Table 5 Effect of mesenteric lymph duct ligation on SOD and MDA in homogenate of two-hit rats (mean ± SD)

Group (n)		Intestine	Kidney	Liver	Lung	Heart
SOD (nkat/g)	Sham (15)	442 255 ± 77 349	273 221 ± 43 009	205 374 ± 35 840	226 212 ± 38 174	300 227 ± 58 011
	Non-ligation (8)	330 399 ± 77 516a	268 720 ± 20 504	204 874 ± 20 838	175 035 ± 44 676	241 882 ± 56 178
	Ligation (11)	467 594 ± 74 182c	256 885 ± 28 172	212 376 ± 35 674	192 205 ± 47 009	339 235 ± 49 510c
MDA (μmol/g)	Sham (15)	2.26 ± 0.65	1.62 ± 0.45	1.66 ± 0.52	1.12 ± 0.25	1.33 ± 0.36
	Non-ligation (8)	3.28 ± 0.58a	2.28 ± 0.16a	2.27 ± 0.28a	1.34 ± 0.33	1.84 ± 0.62
	Ligation (11)	2.81 ± 0.59	2.22 ± 0.58a	2.24 ± 0.34a	1.32 ± 0.20	1.52 ± 0.40

^aP < 0.05 vs sham;

^cP < 0.05 vs non-ligation.