H pylori status and angiogenesis factors in human gastric carcinoma

Table 1 Clinicopathological features and distribution of cagA, vacA and IgG anti-H pylori in a series of 56 gastric cancer patients

Clinicopathological features	n
Sex
Male	37
Female	19
Tumour category
pT1-2-3	28
pT4	28
Location
Antrum	23
Other	33
IgG anti H pylori (ELISA)	39
IgG - (≤ 7 KU/L)	12
IgG + (> 7 KU/L)	27
cagA (PCR)	56
cagA -	32
cagA +	23
NE	1
vacA (PCR)	56
NEG	28
s1m1	10
s2m2	5
s1m2	9
s1m1/s1m2a	1
NE	3

NEG: Negative; NE: Not evaluable;

^a Multiple genome.

Table 2 cag A, vac A, IgG anti-H pylori and angiogenic factors in gastric cancer patients

	IgG anti H pylori		cag A		vac A
	n (%)	n (%)	n (%)	n (%)	n (%)	n (%)
	With IgG- (≤ 7 U/mL)	With IgG+ (> 7 U/mL)	Negative for cagA	Positive for cagA	Cytotoxic strains (s1; m1/2)	Others (absent; s2m2)
TP expression1
02	9 (31)	20 (69)	24 (57)	18 (43)	24 (60)	16 (40)
> 0	2 (25)	6 (75)	8 (80)	2 (10)	8 (80)	2 (20)
p53 expression1
02	8 (25)	24 (75)	27 (60)	18 (40)	28 (65)	15 (35)
> 0	3 (60)	2 (40)	5 (63)	3 (37)	4 (50)	4 (50)
MVD (CD34)1
≤ 372	7 (32)	15 (68)	16 (59)	11 (41)	15 (58)	11 (42)
> 37	3 (21)	11 (79)	16 (64)	9 (36)	17 (71)	7 (29)
VEGF-R1 expression1
≤ 202	6 (29)	15 (71)	16 (59)	11 (41)	14 (54)	12 (46)
> 20	5 (31)	11 (69)	15 (58)	11 (42)	17 (68)	8 (32)
pVEGF levels
≤ 262	9 (50)a	9 (50)a	10 (53)	9 (47)	11 (61)	7 (39)
> 26	2 (13)a	13 (87)a	9 (60)	6 (40)	9 (60)	6 (40)
sVEGF levels
≤ 4322	5 (29)	12 (71)	11 (61)	7 (49)	12 (71)	5 (29)
> 432	6 (32)	13 (68)	9 (47)	10 (53)	9 (47)	10 (53)

sVEGF: Serum VEGF; pVEGF: Plasma VEGF;

¹ % of immunostained cells;

² Cut-off median value of the series;

^aP = 0.026.

Table 3 Association between angiogenic characteristics, markers of H pylori status and clinicopathological features

Biomarkers	Tumor stage			M status
	T1-2-3 (n = 28)	T4 (n = 28)	P	M0 (n = 38)	M1 (n = 18)	P
cagA negative (n = 32)	54	63	NS	58	59	NS
vacA no cytotoxic strains (n = 33)	62	63	NS	58	71	NS
TP negative (n = 42)	93	65	0.035	83	71	NS
p53 negative (n = 46)	78	93	NS	78	100	0.038
low MVD values (n = 27)	56	46	NS	54	44	NS
Low VEGF-R1 expression (n = 27)	56	44	NS	51	47	NS
High IgG levels (n = 27)	65	74	NS	77	54	NS
Low pVEGF levels (n = 19)	74	31	0.03	52	58	NS
Low sVEGF levels (n = 19)	35	67	NS	50	50	NS

NS: Non-significant.