Filtrate of fermented mycelia from Antrodia camphorata reduces liver fibrosis induced by carbon tetrachloride in rats

Table 1 Primer sequences for PCR amplification

mRNA	Primer sequence	Length (bp)
Collagen I	Sense 5' CGA CTA AGT TGG AGG GAA CGG TC 3'	182
	Antisense  5' TGG CAT GTT GCT AGG CAC GAC 3'
TIMP-1	Sense 5' TCC CTT GCA AAC TGG AGA GT 3'	140
	Antisense  5' GTC ATC GAG ACC CCA AGG TA 3'
TGF-b1	Sense 5' TAT AGC AAC AAT TCC TGC CG 3'	162
	Antisense  5' TGC TGT CAC AGG AGC AGTG 3'
GAPDH	Sense 5' CTT CAT TGA CCT CAA CTA CAT GGT CTA 3'	99
	Antisense  5' GATG ACA AGC TTC CCA TTC TCA G 3'

Table 2 Effect of FMAC on plasma albumin concentration and prothrombin time in CCl₄-treated rats

Group	Dose	Albumin	Prothrombin time
	(g/kg per d)	(g/L)	(sec)
Control	–	36.0 ± 1.3	17.7 ± 0.9
CCl4 + H2O	–	22.7±1.0b	36.7 ± 7.2b
CCl4 + FMAC	0.5	23.1 ± 5.1	28.5 ± 9.9
	1	30.7±2.5a	25.1 ± 10.2a

^aP < 0.05 vs CCl₄ + H₂O group; ^bP < 0.01 vs control group.

Table 3 Effect of FMAC on spleen weight, hepatic malondialdehyde and hydroxyproline contents in CCl₄-treated rats

Group	Dose	Spleen	Malondialdehyde	Hydroxyproline
	(g/kg per d)	(g)	(μmol/g protein)	(μg/g tissue)
Control	–	1.1 ± 0.1	1.9 ± 0.1	645.0 ± 64.5
CCl4 + H2O	–	2.7 ± 0.1d	3.9 ± 0.1d	1730.7 ± 258.0d
CCl4 + FMAC	0.5	2.8 ± 0.2	2.7 ± 0.1	1741.5 ± 257.1
	1	2.4 ± 0.2a	2.4 ± 0.6b	1311.5 ± 238.8b

^aP < 0.05, ^bP < 0.01 vs CCl₄ + H₂O group; ^dP < 0.001 vs control group.

Table 4 Effect of FMAC on hepatic mRNA expressions of collagen I, TIMP-1 and TGF-β1 in CCl₄-treated rats

Group	Dose	Collagen I:GAPDH	TGF-β1:GAPDH	TIMP-1:GAPDH
	(g/kg per d)	ratio	ratio	ratio
Control	–	0.68 ± 0.55	0.38 ± 0.18	0.14 ± 0.08
CCl4 + H2O	–	5.63 ± 2.08b	38.03 ± 11.9b	1.70 ± 0.82b
CCl4 + FMAC	0.5	3.94 ± 1.18	28.90 ± 7.22	0.58 ± 0.02
	1	1.78 ± 0.48d	4.26 ± 2.17d	0.34 ± 0.02d

^bP < 0.001 vs Control group; ^dP < 0.01 vs CCl₄ + H₂O group.

Table 5 Effect of FMAC on CCl4-induced liver fibrosis in rats

Group	Dose	Score of hepatic fibrosis					Average
	(g/kg per d)	0	1	2	3	4
Control	–	10	0	0	0	0	0
CCl4 + H2O	–	0	0	3	6	1	2.8 ± 0.6
CCl4 + FMAC	0.5	0	1	3	5	1	2.7 ± 0.8
	1.0	0	4	5	1	0	1.7 ± 0.7b

^bP < 0.01 vs CCl₄ + H₂O group.