Clinical Research
Copyright ©2005 Baishideng Publishing Group Inc.
World J Gastroenterol. May 14, 2005; 11(18): 2748-2753
Published online May 14, 2005. doi: 10.3748/wjg.v11.i18.2748
Table 1 Primer sequences used in RT-PCR analysis.
GeneSize of PCR (bp)Forward primerReverse primer
DPD514TCCTCCAGGTATGCAGTGCCAGTTATGGTGGGCAGGTGGGTT
GAPDH328CAACAGCCTCAAGATCATCAGCTTCTAGACGGCAGGTCAGGTC
GSTP1199TACGGGCAGCTCCCCAAGTTTGCCCGCCTCATAGTTGGTG
MDR1206GTCTTTGGTGCCATGGCCGTATGTCCGGTCGGGTGGGATA
MRP1262CTGACAAGCTAGACCATGAATGTCCTTTGTCCAAGACGATCACCC
MRP2202GCCAGATTGGCCCAGCAAAAATCTGACCACCGGCAGCCT
MRP3452GGGACCCTGCGCATGAACCTGTAGGCAAGTCCAGCATCTCTGG
TS579GAATCACATCGAGCCACTGAAAGTGTTACTCAGCTCCCTCAGA
Table 2 IC50 values of chemotherapeutic agents on CCA cell lines.
DrugIC50 (mmol/L)
Poorly differentiated
Moderately differentiated
Well differentiated
KKU-100KKU-M055KKU-M156KKU-M214KKU-OCA17
Anthracyclins
Doxorubicin2.3±0.8b0.04±0.021.8±1.0b0.25±0.050.84±0.83
Pirarubicin0.27±0.22a0.01±0.010.35±0.06b0.17±0.03b0.13±0.05b
Platinum derivatives
Cisplatin37±3b0.05±0.01222±38b130±12b6.22±0.19b
Carboplatin13±3b0.26±0.01229±36b107±4b10.4±2.7a
Oxaliplatin71.7±38.7a0.11±0.007.0±4.738.0±6.7b6.8±1.3b
Pyrimidine analog
5-Fluorouracil1 018±326d46±42c144±55d29.1±17.6b3.46±0.91
Taxanes
Paclitaxel39.0±6.8b0.0030±0.000110.9±5.8a2.62±0.75a12.5±1.9b
Docetaxel41.2±7.2b0.00002±0.000020.80±0.36a12.4±3.7a1.3±0.3b
Vinca-alkaloids
Vincristine216.2±52.1d2.05±0.68c0.97±0.6485±19d0.82±0.17
Vinorabine2.1±0.2b0.13±0.010.23±0.01a164±1200.64±0.17a
Others
Etoposide416±131b0.04±0.01274±217b54±26a126±88a
Irinotecan23±6a3.41±0.5113.1±2.1b43±9b3.60±1.16
Mitomycin C45±2b0.01±0.00187±0b78±32a5.07±0.17b