Effects of lipopolysaccharides stimulated Kupffer cells on activation of rat hepatic stellate cells

Table 1 Effect of KCCM or LPS on HSC proliferation (mean ± SD, n = 12)

Treatment	A
LPS (1 μg/ml)	0.096 ± 0.003
LPS (10 μg/ml)	0.101 ± 0.004
KCCM1 (unstimulated)	0.123 ± 0.008c
KCCM2 (1 μg/ml LPS-activated)	0.132 ± 0.005a
KCCM3 (10 μg/ml LPS-activated)	0.106 ± 0.010d
KCCM1 + anti-TGF-β1 antibodies	0.109 ± 0.009b
KCCM2 + anti-TGF-β1 antibodies	0.115 ± 0.008d
KCCM3 + anti-TGF-β1 antibodies	0.106 ± 0.010
Control group	0.100 ± 0.003

^aP < 0.01 vs control group;

^bP < 0.01 vs unstimulated KCCM group;

^cP < 0.05,

^dP < 0.01 vs 1 μg/ml LPS-activated KCCM group.

Table 2 Effect of KCCM or LPS on collagen type IV and laminin secretion by HSC (mean ± SD, n = 6)

Treatment	Collagen type IV (μg/L)	Laminin (ng/ml)
LPS (1 μg/ml)	30.53 ± 0.9	57.12 ± 2.00
LPS (10 μg/ml)	29.17 ± 1.36	56.61 ± 2.39
KCCM1 (unstimulated)	34.41 ± 1.22c	62.00 ± 2.23c
KCCM2 (1 μg/ml LPS-activated)	42.39 ± 1.74a	69.40 ± 1.86a
KCCM3 (10 μg/ml LPS-activated)	31.13 ± 0.84d	56.34 ± 2.17d
KCCM1+anti-TGF-β1 antibodies	32.18 ± 1.71b	58.87 ± 2.31b
KCCM2+anti-TGF-β1 antibodies	36.26 ± 0.91d	65.25 ± 1.95d
KCCM3+anti-TGF-β1 antibodies	30.76 ± 1.88	56.16 ± 2.62
Control group	30.34 ± 1.02	57.56 ± 1.56

^aP < 0.01 vs control group;

^bP < 0.01 vs unstimulated KCCM group;

^cP < 0.05,

^dP < 0.01 vs 1 μg/ml LPS-activated KCCM group.

Table 3 TGF-β₁ in LPS-activated and unstimulated KCCM (mean ± SD, n = 6)

Group	TGF-β1(OD)
KCCM1(unstimulated)	0.465 ± 0.075a
KCCM2(1 μg/ml LPS-activated)	0.941 ± 0.114a
KCCM3(10 μg/ml LPS-activated)	0.669 ± 0.093a
Control group	0.089 ± 0.010

^aP < 0.01 vs control group.