Degradation of retinoid X receptor α by TPA through proteasome pathway in gastric cancer cells

Figure 2 Translocation of RXRα from the nucleus to the cyto-plasm induced by TPA. Cells were treated with TPA for differ-ent time periods or CHX (10 g/L) for 3 hrs as required. (A) Cells were immunostained with anti-RXRα antibody followed by corresponding FITC-conjugated anti-IgG secondary anti-body to show RXRα protein. Simultaneously, cells were stained with PI to display the nucleus. The fluorescent image was ob-served under laser-scanning confocal microscope. (B) Nuclear and cytoplasmic fractions were prepared as described in the Materials and Methods. RXRα was revealed by Western blot.