Down-regulation of γ-synuclein in human esophageal squamous cell carcinoma

Figure 1 Simi-quantitative RT-PCR analysis of human γ-synuclein mRNA in human esophageal squamous cell carcinoma. The γ-synuclein fragment (384 bp) was amplified by 20 cycles PCR using the paired primers as indicated in Material and Methods. Five micrograms of total RNA isolated from human ESCC tissues and corresponding normal tissues were used as templates for cDNA synthesis respectively. β-actin was used as an internal control. T: esophageal squamous cell carcinoma tissue, N: corresponding normal tissue, 9706: human ESCC 9706 cell line, (-): negative control, M: 100 bp DNA marker.