Effects of DNA methylation on expression of tumor suppressor genes and proto-oncogene in human colon cancer cell lines

Table 4 Expression of APC gene in SW1116 and Colo-320 cells (the band density)

5-aza-dC treatment	Mock treated	2 μmol/L, 24 h	5 μmol/L, 24 h	10 μmol/L, 24 h	2 μmol/L, 72 h	5 μmol/L, 72 h	10 μmol/L, 24 h
SW1116	786.2	1481.2	782.6	796.9	802.9	1173.5	1236.8
Colo-320	1804.6	2388.2	4055.2	1923.9	1803.0	3197.8	3271.7

The density of each band from RT-PCR in each lane of Figure.4 was normalized to the amount of total RNA as determined by the density of band in RT-PCR for β-actin.