Effects of DNA methylation on expression of tumor suppressor genes and proto-oncogene in human colon cancer cell lines

Table 3 Expression of p16^INK4A gene in SW1116 and Colo-320 cells (the band density)

5-aza-dC treatment	Mock treated	2 μmol/L, 24 h	5 μmol/L, 24 h	10 μmol/L, 24 h	2 μmol/L, 72 h	5 μmol/L, 72 h	10 μmol/L, 24 h
SW1116	1494.7	2055.5	2436.9	3487.3	1592.0	2074.8	2774.0
Colo-320	809.1	860.6	829.2	1298.8	875.7	923.5	1189.6

The density of each band from RT-PCR in each lane of Figure 3 was normalized to the amount of total RNA as determined by the density of band in RT-PCR for β-actin.