Contribution of eIF-4E inhibition to the expression and activity of heparanase in human colon adenocarcinoma cell line: LS-174T

Figure 1 eIF-4E and GAPDH mRNA expression in oligonucle-otide-treated human colon adenocarcinoma cells. AS. anti-sense oligonucleotide-transfected cells; S. sense oligonucleotide-transfected cells; C. cells treated with liposome only as control. LS-174T cells were treated with 2 μM oligonucleotides, accord-ing to the optimal condition of this cell line in the preliminary experimental results. The numbers under each band showed the relative amount of eIF-4E fragments normalized to that of GAPDH by densitometry. The results suggest that antisense oligonucleotides against eIF-4E inhibit eIF-4E mRNA expres-sion in LS-174T cells, and sense oligonucleotides have no in-hibitory effect on eIF-4E mRNA expression.