S-phase delay in human hepatocellular carcinoma cells induced by overexpression of integrin β1

Figure 5 Ser473-phosphorylated form of PKB was decreased, but p27^kip1 protein level was increased concomitantly in SMMC-7721 parental cells treated with the PI3K inhibitor wortmannin. The parental SMMC-7721 cells were starved with serum-free medium for 48 h, then grown in normal medium/10% CBS containing DMSO (as control, 24 h) or 100 nM wortmannin for the indicated times. The amount of DMSO did not exceed 0.1%, which was determined not to damage the cells. Equal amount of wortmannin was added again after grown for 24 h. The level of Ser473-phosphorylated form of PKB (arrow) was declined, but p27^kip1 protein level was elevated with the treatment of wortmannin in SMMC-7721 cells. Results were representative of at least 3 repeated experiments. Abbreviation: DMSO, dimethylsulfoxide; WT, wortmannin.