Novel assay of competitively differentiated polymerase chain reaction for screening point mutation of hepatitis B virus

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Aug 15, 2003 (publication date) through Nov 24, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Viral Hepatitis

World J Gastroenterol. Aug 15, 2003; 9(8): 1743-1746
Published online Aug 15, 2003. doi: 10.3748/wjg.v9.i8.1743

Table 1 Primers and probes for detecting of HBV G1896A variant

Denomination	Sequence (5’→3’)
BIO-PCP	BIO-GAGACTCTAAGGCTTCTCGATACAGAGCTGAGG
PCA	GCAGTATGGTGAGGTGAGCAATGCTCAG
DIG-PCMd	DIG-CTCACGCTACATTGTGTGCCTTGGGTGGCTTCA
DIG-PCMc	DIG-TGTGCCTTGGGTGGCTTCA
FLU-PCWd	FLU-GTCCGTAGTCTCGTTGTGCCTTGGGTGGCTTGG
FLU-PCWc	FLU-TGTGCCTTGGGTGGCTTGG
PCS	CCACCGTGAACGCCCATCAG
PCSc	CCCGAATTCCACCGTGAACGCCCATCAG
PCAc	CCCAAGCTTGCAGTATGGTGAGGTGAGCAATG

BIO: the abbreviation of biotin; DIG: the abbreviation of digoxigenin; FLU: the abbreviation of fluorescein.

Citation: Peng XM, Chen XJ, Li JG, Gu L, Huang YS, Gao ZL. Novel assay of competitively differentiated polymerase chain reaction for screening point mutation of hepatitis B virus. World J Gastroenterol 2003; 9(8): 1743-1746
URL: https://www.wjgnet.com/1007-9327/full/v9/i8/1743.htm
DOI: https://dx.doi.org/10.3748/wjg.v9.i8.1743