Construction of hpaA gene from a clinical isolate of Helicobacter pylori and identification of fusion protein

Figure 4 Expression of HpaA gene in pET32a-hpaA-BL21(DE3) induced by IPTG with different dosages shown in SDS-PAGE. (1: protein marker; 2: non-induced; 3-5: induced with 0.1, 0.5, 1 mmol•l^-1 of IPTG, respectively; 6: the bacterial precipitate induced with 1 mmol•l^-1 of IPTG; 7: the bacterial supernatant induced with 1 mmol•l^-1 of IPTG).