Preparation and characterization of polyclonal antibodies against ARL-1 protein

doi:10.3748/wjg.v9.i7.1455

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Jul 15, 2003 (publication date) through Dec 24, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Liver Cancer

World J Gastroenterol. Jul 15, 2003; 9(7): 1455-1459
Published online Jul 15, 2003. doi: 10.3748/wjg.v9.i7.1455

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Figure 3 The transformed pGEX-4T-1(His)₆C-ARL BL21 induced by 0. 05 mM isopropyl-1-thio-β-D-galactopyranoside. Lane 1: BL21 transformed with pGEX-4T-1(His)₆C, no IPTG induced; Lane 2: BL21 transformed with pGEX-4T-1(His)₆C, IPTG induced, GST (26KD) was expressed; Lane 3: BL21 transformed with pGEX-4T-1(His)₆C-ARL, no IPTG induced; Lane 4: BL21 transformed with pGEX-4T-1(His)₆C-ARL, IPTG induced, recombinant protein ARL-GST (about 60.8 KD) was expressed; Lane 5: supernatant; Lane 6: pellet; Lane 7: purified ARL-GST; Lane 8: mid-range protein molecular weight markers.

Citation: Jin JF, Yuan LD, Liu L, Zhao ZJ, Xie W. Preparation and characterization of polyclonal antibodies against ARL-1 protein. World J Gastroenterol 2003; 9(7): 1455-1459
URL: https://www.wjgnet.com/1007-9327/full/v9/i7/1455.htm
DOI: https://dx.doi.org/10.3748/wjg.v9.i7.1455