Preparation and characterization of polyclonal antibodies against ARL-1 protein

doi:10.3748/wjg.v9.i7.1455

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Jul 15, 2003 (publication date) through Nov 27, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Liver Cancer

World J Gastroenterol. Jul 15, 2003; 9(7): 1455-1459
Published online Jul 15, 2003. doi: 10.3748/wjg.v9.i7.1455

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Figure 1 Recombinant plasmid pGEX-4T-1(His)₆C-ARL was identified by restrictive endonuclease and polymerase chain reaction (1. 5% agarose electrophoresis). Lane 1: The recombinant plasmid pGEX-4T-1(His)₆C-ARL digested by EcoR I only, about 5.9 kb fragment could be seen; Lane 2: The recombinant plasmid pGEX-4T-1(His)₆ C-ARL digested by EcoR I and Xho I, two fragments of 5.0 kb and 950 bp could be seen; Lane 3: pGEX-4T-1(His)₆C-ARL being template, 950 bp was amplified by polymerase chain reaction; Lane 4: positive control of PCR; Lane 5: negative control of PCR; Lane 6: GeneRuler^TM 100 bp DNA ladder plus.

Citation: Jin JF, Yuan LD, Liu L, Zhao ZJ, Xie W. Preparation and characterization of polyclonal antibodies against ARL-1 protein. World J Gastroenterol 2003; 9(7): 1455-1459
URL: https://www.wjgnet.com/1007-9327/full/v9/i7/1455.htm
DOI: https://dx.doi.org/10.3748/wjg.v9.i7.1455