Gene-expression analysis of single cells-nested polymerase chain reaction after laser microdissection

Figure 3 Amplification results from nested RT-PCR for cDNA of single cells after laser microdissection. A: Amplification results of β-actin-outer primers. B: Amplification results of β-actin-inner primers. M: DNA markers (upper to lower: 2000, 1000, 900, 800, 700, 600, 500, 400, 300, 200, and 100 bp). 1: PCR positive control; 2: one complete section; 4, 6, 8 and 10 are 200, 100, 10 and 1 cell(s), respectively; 3, 5, 7, 9 and 11 are negative controls of 2, 4, 6, 8 and 10; 12: PCR negative control.