Gene-expression analysis of single cells-nested polymerase chain reaction after laser microdissection

Figure 1 Examples of laser microdissection in colon tissues (hematoxylin and eosin, magnification × 200). Single cells were picked by combination laser-manipulated microdissection (LMM) and laser pressure catapulting (LPC). A, C, E, G: Before Laser microdissection. B, D, F, H: Using LMM, the laser precisely circumcised selected cells, yielding a cut gap between selected and non-selected areas. Then the selected cells were catapulted using the LPC technique. A-F: Typical images before and after LMM and LPC. G, H: Laser microdissection could also be used to cut out the nucleus of a selected cell.