Rapid mitogen-activated protein kinase by basic fibroblast growth factor in rat intestine after ischemia/reperfusion injury

Figure 1 Immunohistochemical staining of phosphorylated p42/p44 MAPK in intestinal biopsies in rats after ischemia/reperfusion injury (SP × 200). A: Negative control of p42/p44 MAPK staining. There was no positive expression signal in this group; B: The expression of phosphorylated p42/p44 in intestinal biopsies in the saline control group 2 h after reperfusion. The activated p42/p44 MAPK expression was localized in the cytoplasm and nuclei of villus cells and in the nuclei of crypt cells, mainly in the epithelium and villus cells; C: Phosphorylated p42/p44 staining in the bFGF antibody pre-treated group. The number of positive cells and intensity in this group were weaker compared with those in the saline control and bFGF treated groups; D: The expression of phosphorylated p42/p44 in the bFGF treated group 2 h after reperfusion. The activated p42/p44 MAPK expression was localized in the cytoplasm and nuclei of villus cells and in the nuclei of crypt cells, mainly in the epithelium and villus cells. The number of positive cells in this group was more than that in the bFGF antibody pre-treated group. ISH × 400.