Synergistic antitumor effect of TRAIL and doxorubicin on colon cancer cell line SW480

Figure 3 Apoptosis assay by flow cytometry. SW480 cells were treated with doxorubicin (0.86 μmol•l^-1) alone, TRAIL (100 μg•l^-1) alone or a combination of them for 24 h. The apoptosis-inducing effect was quantified by PI stain flow cytometry assay. A: There was no hypodiploid DNA peak before G₁ phase which was similar to normal cells when treated with TRAIL (100 μg•l^-1) or doxorubicin (0.86 μmol•l^-1) alone. B: There was an obvious hyperdiploid DNA peak before G₁ phase in combination treatment, with apoptotic rate of 76.82% ± 1.93%. It was in accordance with cytotoxicity by MTT assay.