Copyright
©The Author(s) 2003.
World J Gastroenterol. Jun 15, 2003; 9(6): 1347-1351
Published online Jun 15, 2003. doi: 10.3748/wjg.v9.i6.1347
Published online Jun 15, 2003. doi: 10.3748/wjg.v9.i6.1347
Relevant characteristics | Source | |
Strains | ||
S. flexneri 2a, 2457T | Wild type, Nalr | Maurelli AT |
E. coli DH5α | SupE44, ΔlacU169 (Φ80 lacZΔM15), hsdR17, recA1, endA1, gyrA96, thi-1, relA1, Nalr | Our lab |
S. cerevisiae AH109 | MATa, trp1-901, leu2-3, ura3-52, his3-200, gal4Δ, gal80Δ, LYS2::GAL1UAS-GAL1TATA-HIS3, GAL2UAS-GAL2TATA-ADE2, URA3::MEL1UAS-MEL1TATA-LacZ | Clontech |
Plasmids | ||
pGBKT7 | GAL4 DNA-BD, TRP1, c-Myc epitope tag, Kmr Clontech | |
pGBKT-ipaC | GAL4 DNA-BD fusion of IpaC, TRP1, c-Myc epitope tag, Kmr | This study |
pACT2 | GAL4 DNA-AD, HA epitope, LEU2, Apr | Clontech |
pLAM5'-1 | GAL4 DNA-BD fusion of the human lamin C, TRP1, a false-positive detection plasmid, Apr | Clontech |
p1 | A positive library plasmid | This study |
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Citation: Yao X, Wang HL, Shi ZX, Yan XY, Feng EL, Yang BL, Huang LY. Identification of RanBMP interacting with
Shigella flexneri IpaC invasin by two-hybrid system of yeast. World J Gastroenterol 2003; 9(6): 1347-1351 - URL: https://www.wjgnet.com/1007-9327/full/v9/i6/1347.htm
- DOI: https://dx.doi.org/10.3748/wjg.v9.i6.1347