Viral Hepatitis
Copyright ©The Author(s) 2003.
World J Gastroenterol. May 15, 2003; 9(5): 978-983
Published online May 15, 2003. doi: 10.3748/wjg.v9.i5.978
Figure 2
Figure 2 Detection and time course of HBV RNA in liver. Poly A(+) RNA from livers were isolated, reverse transcribed and amplified by PCR as described in Materials and Methods to generate a 355 bp fragment. Lane 1, molecular size markers; lane 2, normal untreated rat liver; lane 3, Huh7 human liver cell line; lane 4, HepG2 2.2.15 cells which constitutively pro-duce infectious HBV particles. Livers from rats after tolerization and transplantation with human hepatocytes and subsequent inoculation with HBV after 1 week, lane 5; 6 weeks, lane 6, and 14 weeks, lane 7.