Response of human REV3 gene to gastric cancer inducing carcinogen N-methyl-N’-nitro-N-nitrosoguanidine and its role in mutagenesis

Figure 1 The detection system of untargeted mutation occur-ring on undamaged DNA template. Cells were pretreated by MNNG, and then intact and undamaged shuttle plasmid pZ189 was transfected into cells after removing MNNG. After repli-cation for 48 h in cells, replicated pZ189 plasmid was rescued and then transformed to host bacterial MBM7070 to screen pZ189 mutants.