Repression of allo-cell transplant rejection through CIITA ribonuclease P+ hepatocyte

Figure 1 Cleavage of CIITA substrate by M1-GS RNA in vitro (A): The mould plate RNA of CIITA pGM-3176 (3176-3560) include the cutting point of only M1-3408-GS, not M1-452-GS. (B): Schematic representation of targeting the CIITA mRNA by M1-RNA: GS encoding 12 or 11 nucleotides complemen-tary to CIITA was covalently linked to the 3’ end of M1-452-GS or M1-3408-GS. So M1-452-GS and M1-3408-GS specifily cut CIITA on the site of 452 and 3408 respectivly. (C): Sequence-specific cleavage of pGM-3176 substrate by M1-3408-GS: auto-radiograph of transcripts of M1-452-GSC (lane 1), M1-3408-GS (lane 2) and pGM-3176 (lane 3), and pGM-3176 substrate was incubated either with M1-3408-GS (lane 4), or with M1-452-GS (lane 5). So only M1-3408-GS (not M1-452-GS) could cleave pGM-3176.