A small yeast RNA inhibits HCV IRES mediated translation and inhibits replication of poliovirus in vivo

Figure 6 IRNA forms a gel-retarded complex which is inhibited by 5’ UTR of HCV RNA. ³²P-labeled IRNA was incubated with RRL extract in the absence (lane 2) or presence of an unla-beled 25- or 50-fold molar excess of IRNA (lane3 and lane 4, respectively) or 5’ UTR (lane 5) or of a 50-fold molar excess of nonspecific RNA (lane 6). Protein-RNA complexes were analyzed on a nondenaturing gel. In lane 1, RRL extract was not added. FP: free probe; C: complexes.