Hormonal regulation of dipeptide transporter (PepT1) in Caco-2 cells with normal and anoxia/reoxygenation management

Figure 4 Uptake of 1 mM cephalexin in Caco-2 cells. Caco-2 cells cultured in the multiwell dishes (24 pore) were cultured for 4 d with 34 nM rhGH. After washing the monolayers, the transport medium with 1 mM cephalexin was added to the pores and uptake was measured at 0, 5, 10, 30, 60, 90, and 120 min. At the end of the incubation period, cell monolayers were washed with PH7.4 PBS to remove any extracellular cephalexin. Accumulated concentrations of cephalexin were determined by HPLC after the cells were lysed. The differences were significant (P = 0.0223).