Hormonal regulation of dipeptide transporter (PepT1) in Caco-2 cells with normal and anoxia/reoxygenation management

Figure 3 Transport of 1 mM cephalexin in Caco-2 cells. Caco-2 cells were cultured with rhGH in apical side for 4 d. The controls were incubated with PH7.0 culture medium without rhGH both in apical side and basolateral side. All the mono-layers were washed in each experiment. The apical chambers were then filled with 1.5 ml solution containing 1 mM cephalexin. Transport was examined within the time range of 0, 5, 10, 30, 60, 90, and 120 min. At the end of the incubation period, the inserts of Transwell were removed and the trans-port samples were obtained from the basolateral chambers. The concentrations of cephalexin of the transport samples were determined by HPLC. The apical-to-basolateral transport of cephalexin was significantly increased compared with the con-trols (P = 0.0045).