Gene expression profiles of hepatoma cell line HLE

Figure 3 Northern blot analysis of 2 genes to confirm the Atlas human cancer cDNA expression array. Four paired cases were used to determine these genes expression patterns. Twenty µg RNA was analyzed on a 1.2% denaturing agrose gel and trans-ferred onto a nylon membrane. ³²P-labeled cDNA probes for these genes were hybridized to the RNA-blotted membranes. After stringent washes, membranes were exposed to X-ray film overnight at -70 °C. The same membranes were rehybridized with human β-actin for an RNA loading control (N, adjacent normal liver tissue; C, human HCC tissue).