Translocation of annexin I from cellular membrane to the nuclear membrane in human esophageal squamous cell carcinoma

Figure 1 The localization of annexin I in normal esophageal epithelium. The normal esophageal epithelia on the paraffin-embed-ded tissues were detected with anti-annexin I monoclonal antibody and imaged with TRITC-conjugated goat anti-mouse antibody, and then observed under a fluorescence microscope (a, d, e, f and g). PBS was used as a negative control (a 50 ×), the tissue section was viewed under a Nomarski interference-contrast microscopy (b 50 ×) and the additional H & E staining was also performed (c 200 ×). The localization of annexin I protein in normal esophageal epithelium was shown from d to g (d 200 ×; e 400 ×; f 400 × and g 400 ×). The orientation of the bottom left corner was the basal membrane of the esophageal mucosa.