Effects of hepatitis B virus infection on human sperm chromosomes

Table 2 Analytical results of sperm chromosomes of the tested subjects

Sub-jects	Number of metaphase spreads observed	Number (%)of analyzable metaphase spreads	Number(%) of normal sperm karyotypes	Number of abnormal sperm								Rates (%) of anormal sperm	FISH Re-sults
				With aneu-ploidy	With structural aberrations
					g	r	tr	dic	pul	ace	del
Hepatitis
1	73	23 (31.5)	20 (87.0)	1	0	0	0	1	0	1	0	13.0	-
2	69	20 (29.0)	15 (75.0)	1	1	0	2	0	0	0	1	25.0	-
3	72	24 (33.3)	22 (91.7)	0	1	0	0	0	1	0	0	8.3	-
4	68	29 (42.6)	25 (86.2)	0	0	1	0	2	0	1	0	13.8	-
5	86	30 (34.9)	28 (93.3)	1 (1)a	0	1	1a	0	0	0	0	6.7	-
6	113	43 (38.1)	30 (69.8)	5 (2)a	4 (1)b	2 (1)a	2 (1)a	0	2	1b	0	30.2	+
7	82	19 (23.2)	19 (100 )	0	0	0	0	0	0	0	0	0.0	-
8	61	23 (37.7)	20 (87.0)	0	0	1	0	1	1	0	0	13.0	-
9	48	12 (25.0)	11 (91.7)	1	0	0	0	0	0	0	0	8.3	-
Total	672	223 (33.2)	190 (85.2)	9 (3)a	6 (1)b	5	5	4	4	3	1	14.8	-
Controls
1	57	31 (54.3)	29 (93.5)	0	0	1	0	0	0	1	0	6.5	-
2	61	21 (34.4)	21 (100 )	0	0	0	0	0	0	0	0	0.0	-
3	50	19 (38.0)	19 (100 )	0	0	0	0	0	0	0	0	0.0	-
4	36	17 (47.2)	16 (94.1)	1	0	0	0	0	0	0	0	5.9	-
5	42	28 (66.7)	26 (92.9)	0	1	0	0	1	0	0	0	7.1	-
Total	246	116 (47.2)	111 (95.7)	1	1	1	0	1	0	1	0	4.3	-

g = Gap; r = Ring; tr = Triradial; dic = Dicentric chromosome; pul = Pulverization; ace = Acentric fragment; del = Deletion

^aFor containing both numerically and structurally chromosomal aberrations simultaneously

^bFor containing gap and acentric fragment chromosome aberrations simultaneously