Follow up of infection of chacma baboons with inoculum containing a and non-a genotypes of hepatitis B virus

Table 2 Polymerase chain reaction cycling profiles

		Amplification conditions			Sizeb
		Denaturation	Annealing	Extension
Double-round	PCR1	94°C 30 sec	62°C 40 sec	72°C 80 sec	812 bp
PCR
	PCR2	94°C 30 sec	57°C 50 sec	72°C 50 sec	170 bp
Triple-round	PCR1	94°C 30 sec	62°C 40 sec	72°C 80 sec	812 bp
PCR
	PCR2	94°C 30 sec	48°C 60 sec	72°C 50 sec	642 bp
	PCR3	94°C 30 sec	50°C 60 sec	72°C 50 sec	134 bp

PCR1: The first round polymerase chain reaction; PCR2: The second round polymerase chain reaction; PCR3: The triple round polymerase chain reaction. (+) sense; (-) anti-sense. ^aDenotes the nucleotide position of hepatitis B virus adw ge-nome (GenBank accession #V00866) where the EcoRI cleavage site is position 1.

^bSize of the amplicons in base pairs.