Follow up of infection of chacma baboons with inoculum containing a and non-a genotypes of hepatitis B virus

doi:10.3748/wjg.v9.i4.731

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Apr 15, 2003 (publication date) through Feb 21, 2025

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Viral Hepatitis

World J Gastroenterol. Apr 15, 2003; 9(4): 731-735
Published online Apr 15, 2003. doi: 10.3748/wjg.v9.i4.731

Table 1 Oligonucleotide primers

		Primer	Position^a	Sequence
		1687 (+)	1687-1706	5’CGACCGACCTTGAGGCATAC3’
Double-	PCR1	2498 (-)	2498-2477	5’AAGCCCAGTAAAGTTTCCCACC3’
round		2267 (+)	2267-2284	5’GGAGTGTGGATTCGCACT3’
PCR	PCR2	2436 (-)	2436-2419	5’TGAGATCTTCTGCGACGC3’
		1687 (+)	1687-1706	5’CGACCGACCTTGAGGCATAC3’
Triple-	PCR1	2498 (-)	2498-2477	5’AAGCCCAGTAAAGTTTCCCACC3’
round		1795 (+)	1795-1812	5’TGGTCTGTCGACCAGCAC3’
PCR	PCR2	2436 (-)	2436-2419	5’TGAGATCTTCTGCGACGC3’
		2267 (+)	2267-2284	5’GGAGTGTGGATTCGCACT3’
	PCR3	2400 (-)	2400-2382	5’CTGCGAGGCGAGGGAGTT3’

PCR1: The first round polymerase chain reaction; PCR2: The second round polymerase chain reaction; PCR3: The triple round polymerase chain reaction. (+) sense; (-) anti-sense.

^aDenotes the nucleotide position of hepatitis B virus adw ge-nome (GenBank accession #V00866) where the EcoRI cleavage site is position 1. ^bSize of the amplicons in base pairs.

Citation: Baptista M, Kramvis A, Jammeh S, Naicker J, Galpin JS, Kew MC. Follow up of infection of chacma baboons with inoculum containing a and non-a genotypes of hepatitis B virus. World J Gastroenterol 2003; 9(4): 731-735
URL: https://www.wjgnet.com/1007-9327/full/v9/i4/731.htm
DOI: https://dx.doi.org/10.3748/wjg.v9.i4.731