Preparation and identification of anti-transforming growth factor β1 U1 small nuclear RNA chimeric ribozyme in vitro

Figure 6 The kinetic of cleavage reaction for U1Rz803. (A) Specific cleavage of target RNA by U1Rz803 for the kinetic of U1Rz803 in vitro. U1Rz803 concentration was 5 nmol·L^-1, sub-strate concentration was 10, 20, 40, 80, 160 nmol·L^-1 from left to right. (B) Lineweaver-Burk kinetic plots of cleavage reaction for U1Rz803 in vitro. U1Rz803 concentration was 5 nmol·L^-1, substrate concentration was 160, 80, 40, 20, 10 nmo·L^-1 for each dot from left to right. Reactions were performed at 37 °C for 20 minutes.