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©The Author(s) 2003.
World J Gastroenterol. Mar 15, 2003; 9(3): 578-583
Published online Mar 15, 2003. doi: 10.3748/wjg.v9.i3.578
Published online Mar 15, 2003. doi: 10.3748/wjg.v9.i3.578
Table 3 Summary of multiplex PCR analysis of THH-induced HPRT mutants in HL-60 cells
| Categories of mutation | Number analyzed | Number showing PCR changes | Percentage deleted | Number showing no change | |
| Complete deletion | Partial deletion | ||||
| Spontaneous | 13 | 0 | 1/13 (7.7%) | 7.7% | 12/13 (92.3%) |
| ENU (μg/mL) | |||||
| 12.5 | 8 | 1/8 (12.5%) | 4/8 (50.0%)a | 62.5%b | 3/8 (37.5%)a |
| 25.0 | 10 | 2/10 (20.0%) | 5/10 (50.0%)a | 70.0%b | 3/10 (30.0%)b |
| 50.0 | 10 | 2/10 (20.0%) | 6/10 (60.0%)b | 80.0%b | 2/10 (20.0%)b |
| 100.0 | 12 | 4/12 (33.3%)a | 6/12 (50.0%)a | 83.3%b | 2/12 (16.7%)b |
| 200.0 | 19 | 7/19(36.8%)a | 10/19 (52.6%)a | 89.4%b | 2/19 (10.6%)b |
| γ-rays (Gy) | |||||
| 0.5 | 7 | 1/7 (14.3%) | 1/7 (14.3%) | 28.6% | 5/7 (71.4%) |
| 1.0 | 13 | 3/13 (23.1%) | 5/13 (38.5%) | 61.5%b | 5/13 (38.5%)b |
| 2.0 | 10 | 3/10 (30.0%)a | 3/10 (30.0%) | 60.0%b | 4/10 (40.0%)b |
| 4.0 | 17 | 7/17 (41.2%)b | 6/17(35.3%) | 76.5%b | 4/17 (23.5%)b |
- Citation: Liu SX, Cao J, An H, Shun HM, Yang LJ, Liu Y. Analysis of spontaneous, gamma ray- and ethylnitrosourea-induced hprt mutants in HL-60 cells with multiplex PCR. World J Gastroenterol 2003; 9(3): 578-583
- URL: https://www.wjgnet.com/1007-9327/full/v9/i3/578.htm
- DOI: https://dx.doi.org/10.3748/wjg.v9.i3.578