Targeted ribonuclease can inhibit replication of hepatitis B virus

Figure 3 Confirmation of transgenes expression in transfected 2. 2.15 cell line. Forty-eight h after the transfection, total RNA was isolated from transfected 2.2.15 cells and RT-PCR was performed to confirm the expression of transgenes. The RT-PCR products were then electrophoresed in 1.2% agarose gel. Lanes 1-5 represent the RT-PCR results for total RNA isolated from 2.2.15 cells transfected by p/TN, p/TNmut, p/HBVc, p/hEDN, and pcDNA3.1 (-), respectively. Lanes 6-10 represent controls corresponding to lanes 1-5, respectively, in which reverse transcriptase was omitted in RT-PCR. M: DNA Marker (2000, 1000, 750, 500, 250, 100bp from top to bottom).