Targeted ribonuclease can inhibit replication of hepatitis B virus

Figure 2 Generation of hEDNmut gene by sequential PCR. For PCR 1 and 2, pUC18/hEDN was used as template. Primers pair M1 and N2 were used in PCR 1 and N1 and M2 in PCR 2. For PCR 3, the mixture of PCR 1 and 2 product was used as template and M1 and M2 as primers. The black dots in the figure denote the nucleotides introducing the Lys113→Arg mutation which eliminates the ribonuclease activity.