Identification of alkA gene related to virulence of Shigella flexneri 2a by mutational analysis

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Dec 15, 2003 (publication date) through Nov 2, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Dec 15, 2003; 9(12): 2720-2725
Published online Dec 15, 2003. doi: 10.3748/wjg.v9.i12.2720

Table 1 Strains and plasmids

Strains and plasmids	Characteristics	Source (reference)
Strains
DH5α	endA1 hsdR17(r_k^-m_k⁺) supE44 thi-1 recA1 gyrA(Nal^r)	Our lab
	RelA1 △ lacIZYA-argF) U169 deoR (φ80dlac △(lacZ)M15)
S_17-1λpir	thi-1 thr leu tonA lacY supE recA::RP4-2-Tc::Mu, Sm^r, λpir	Guzman CA
MC1061	hsdR2 mcrB araD139 △ (ara ABC-leu)7679 △ lacX74 galU galK rpsL thi, Sm^r	Our lab
2457T	S. flexneri 2a wild type, Nal^r	Maurelli AT
2457T05	a derivative strain of 2457T, contained araBp-gam-bet-exo, Nal^r	This study
2457T028D	△ alkA, derivative strain of 2457T, Nal^r, Km^r	This study
Plasmids
pMD18-T	a derivative constructed from pUC18, Ap^r	TaKaRa
pXL275	a mobile and suicide plasmid, ori R6K, mob RK2, sacBR, Cm^r	Rui et al[17]
pKD46	oriR101, repA101(ts), araBp-gam-bet-exo, Ap^r	CGSC^a
pXLkd46	pXL275 derivative, inserted a fragment containing araBp-gam-bet-exo, Cm^r	This study
pMDKm05	pMD18-T derivative, inserted Km^r gene, Km^r, Ap^r	Our lab
pMD028	pMD18-T derivative, inserted alkA gene, Ap^r	This study
pMD028pKm	pMD18-T derivative, inserted a cassette (5’alkA end-Km^r-3’alkA end), Km^r, Ap^r	This study

^aCGSC: E. coli Genetic Stock Center.

Citation: Shi ZX, Wang HL, Hu K, Feng EL, Yao X, Su GF, Huang PT, Huang LY. Identification of alkA gene related to virulence of Shigella flexneri 2a by mutational analysis. World J Gastroenterol 2003; 9(12): 2720-2725
URL: https://www.wjgnet.com/1007-9327/full/v9/i12/2720.htm
DOI: https://dx.doi.org/10.3748/wjg.v9.i12.2720